Royal Agricultural University Cirencester, UK

Study acid buffering capacity of EQUINE74 GASTRIC


Journal of Equine Veterinary Science 34 (2014) 391–397
Original Research

An In Vitro Investigation into the Effects of a Marine-Derived, Multimineral Supplement in Simulated Equine Stomach and Hindgut Environments

Meriel Moore-Colyer PhD a,*, Denise M. O’Gorman PhD b, Katherine Wakefield BSc a

Equine 74 Gastric has a high acid-buffering enabled by its high calcium content. The unique sponge-like structure of the Seaweed Lithothamnium Calcareum helps to absorb the acid in the horse’s stomach.
The buffering activity of Equine 74 Gastric was researched by the Royal Agricultural College in Cirencester, UK. This study explored the buffering of acid production by Equine 74 Gastric. In order to do so, the production of lactic acid and the pH value were measured in vitro. The results indicated that the buffer capacity of Equine 74 Gastric kept the pH value constant in all treatments, despite the increase in lactic acid production, and a decreasing pH value.
This feature has important nutritional benefits and is helpful in maintaining the gastric mucosal integrity of the stomach – especially for horses that are fed on high value concentrates. The nutritional benefits when feeding Equine 74 Gastric can be multifactorial.
The research, peer-reviewed and published by experts, supports many assumptions about this unique and natural supplement feed, that is ideal for almost all horses.

a*) School of Agriculture, Royal Agricultural College, Cirencester, Gloucestershire, United Kingdom
b*) Marigot Ltd, Strand Farm, Currabinny, Carrigaline, Co. Cork, Ireland

Equine Gastric Ulceration Syndrome

Dr Tim Brazil BVSc, PhD Cert EM (Internal Medicine) DECEIM MRCVS
European Specialist in Equine Internal Medicine
Equine Practitioner, Moreton-in-Marsh, Gloucestershire, UK

The initial trial work with Equine 74 Gastric, performed in horses engaged in race and eventing training, in Eire is most encouraging. Wisely only horses with mild (Grade 2) Equine Gastric Ulceration Syndrome were included in the trial. Those with more severe disease would have warranted acid suppressant therapy to optimise their health and performance. However the response of low grade ulcers to inclusion of Equine 74 Gastric in feed for 4 weeks demonstrated healing whilst the horses remained in competitive work.


Management of the performance horse often incorporates meal feeding of highly digestible starches and reduced access to high-fiber forage. Such regimens are associated with equine gastric ulceration syndrome (EGUS) and can alter hindgut homeostasis. Infeed buffering of gastric contents and promotion of energy derivation from high-fiber forage in the hindgut are therefore desirable properties of a nutritional supplement.

A marine derived, multimineral supplement with known buffering properties containing calcium, magnesium, and short-chain fructo-oligosaccharides (scFOS) was tested under in vitro simulations of equine stomach and hindgut conditions. Six fiber:concentrate diets were incubated for 4 hours with or without the supplement at 37C in pepsin HCl solution adjusted to pH 4.1 and 2.6. pH was measured at 1, 2, and 4 hours post incubation.

Highest overall pH values were observed with the high cereal feeds; however, the supplement significantly increased (P < .001) the pH across all feeds by 0.17 and 0.19 for feeds incubated at pH 4.1 and 2.6, respectively. A gas production technique was used to measure the fermentation of four fiber:concentrate diets with and without additional supplement, using equine feces as the microbial inoculum. Addition of the supplement decreased (P < .05) the lag time and increased the initial fermentation rate, although as the incubation continued, this effect was reduced. These results demonstrate that the supplement had a significant buffering action for 4-6 hours under simulated in vitro stomach digestion conditions and also stimulated in vitro hindgut fermentation activities. 2014 Elsevier Inc. All rights reserved. A r t i c l e i n f o, Article history: Received 23 May 2013 Received in revised form 3 July 2013 Accepted 31 July 2013 Available online 26 September 2013 Tim has been graduated in Liverpool University in 1988. He then spent 11 years dividing his time between private practice and University teaching hospital jobs in Canada, Liverpool & Edinburgh. In 1995 he was the first person to be awarded the Royal College of Veterinary Surgeons Certificate in Equine Internal Medicine. He was awarded a PhD from Edinburgh University in 1999 for research into lung disease in horses and became a Diplomate of the European College of Equine Internal Medicine in 2005.