An in-vitro investigation demonstrates the Acid Buffering Capacity of Equine 74                                Royal Agricultural University, Dr Meriel Moore-Colyer PhD  


Journal of Equine Veterinary Science 34 (2014) 391–397 Original Research

Meriel Moore-Colyer PhD, Denise M. O’Gorman PhD , Katherine Wakefield BSc

This research evaluated the potential for Equine74 to increase fermentation and to buffer acid production by measuring gas production, lactic acid production and pH levels in-vitro. The in-vitro techniques used were designed to simulate hindgut conditions in the horse. Pre-digestion conditions were incorporated to simulate the enzyme activity and acid conditions of the foregut.

Two commercial diets rich in fibre or concentrate were combined to give a total of four different diets for the trial.

1. High fibre diet +/- Equine74Gastric
2. High fibre (70%): high concentrate (30%) mix +/-Equine74Gastric
3. High fibre (30%): high concentrate (70%) mix +/-Equine74Gastric
4. High concentrate diet +/-Equine74Gastric

• Gas production
• Lactic acid production
• pH level











• Increased concentrate ratios in the diets resulted in increased fermentation as measured by increased lactate levels, increased gas production and by a drop in pH.

• The addition of Equine74 Gastric numerically stimulated fermentation as measured by lactate production (1.87mmol/L) and gas production.

• The buffering capacity of Equine74 Gastric kept pH levels constant in all diets despite the rise in lactate production.

• Incubation of Equine74 Gastric with solutions designed to mimic foregut acid hydrolysis did not prevent the beneficial action of the buffering capacity of Equine74Gastric.

•Equine 74 Gastric increased fermentation rates
•Equine 74 Gastric prevented a drop in pH
•Equine 74 Gastric was not degraded by simulated foregut      hydrolysis